Composite

Part:BBa_K4729702

Designed by: Yasoo Morimoto   Group: iGEM23_Marburg   (2023-10-10)


Ptac + VirG N54D + BBR1

a

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2222
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 262
    Illegal BamHI site found at 1839
    Illegal BamHI site found at 2131
    Illegal XhoI site found at 227
    Illegal XhoI site found at 1778
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 111
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Khan, S. R., Gaines, J., Roop, R. M., II, & Farrand, S. K. (2008). Broad-Host-Range Expression Vectors with Tightly Regulated Promoters and Their Use To Examine the Influence of TraR and TraM Expression on Ti Plasmid Quorum Sensing. In Applied and Environmental Microbiology (Vol. 74, Issue 16, pp. 5053–5062). American Society for Microbiology. https://doi.org/10.1128/aem.01098-08

Seiler, V. N. (2023). Improved inducible switches for the implementation of genetic tools in Sinorhizobium meliloti and Ensifer adhaerens (Master's thesis, Philipps-University Marburg)


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